Abstract

Weeding is a practice of primary importance both in conventional agriculture where the intensive use of herbicides can represent a significant environmental risk and in organic farming where this practice is prohibited. Therefore, the need to identify alternative means of controlling weeds is evident. In this respect, allelopathy proves to be a useful tool to be integrated with conventional agronomic techniques for the management of infesting flora. In this work, we investigated the phytotoxic potential of Achillea moschata Wulfen (Asteraceae) against Echinochloa oryzoides (Ard.) Fritsch and Lolium multiflorum Lam., two noxious plants for crops such as rice and wheat. Preliminary anti-germination assays were carried out in controlled conditions by testing both the powder (0.25 g) and the aqueous extract (1%, 10% and 20%) from the A. moschata aerial parts. The obtained results showed that the powder was more effective than the extract in inhibiting seed germination (up to –81.6% vs –48.8% at 20% concentration) and seedling development (up to –99.4% vs –75.9% at 20% concentration) of both target species, although L. multiflorum was more susceptible than E. oryzoides to treatments. Furthermore, the chemical composition of the two used matrices was characterized by Solid-Phase Microextraction (SPME) sampling technique and Gas Chromatograph/Mass spectrometer (GC/MS) analyses. Camphor (25.8% and 49.9%) and 1,8 cineole (25.9% and 20.7%) were the main constituents in the samples, followed by bornyl acetate (6.7%) in the powder and fragranol (10.5%) in the aqueous extract.

Keywords

Achillea genus; anti-germination activity; biocontrol; phytotoxicity; weed management; SPME-GC-MS

References

1.       Li Z.R.; Amist N.; Bai L.Y. Allelopathy in sustainable weeds management. Allelopathy J. 2019, 48, 109-138.

2.       Cai X.; Gu M. Bioherbicides in organic horticulture. Horticulturae 2016, 2, 3.

3.       Godlewska, K.; Ronga, D.; Michalak, I. Plant extracts - importance in sustainable agriculture. Ital. J. Agron. 2021, 16.

4.       Vitalini, S.; Orlando, F.; Palmioli, A.; Alali, S.; Airoldi, C.; De Noni, I.; Vaglia, V.; Bocchi, S.; Iriti, M. Different phytotoxic effect of Lolium multiflorum Lam. leaves against Echinochloa oryzoides (Ard.) Fritsch and Oryza sativa L. Environ. Sci. Pollut. Res. 2020, 27, 33204–33214.

5.       Vitalini, S.; Iriti M.; Orlanso, F.; Garzoli, S. Chemical volatile composition and phytotoxic potential of Daphne gnidium L. leaves. Sustain. Chem. Pharm. 2022, 25, 100607.

6.       Pignatti, S. Flora d’Italia, Edagricole, Bologna; Vol. 3: p.79; 1982.

7.       Al-Mudaris, M. Notes on various parameters recording the speed of seed germination’. Tropenlandwirt 1998, 99, 147–154.

8.       Ellis, R.A.; Roberts, E.H. The quantification of ageing and survival in orthodox seed. Seed Sci. Technol. 1981, 9, 373–409.

9.       Abdul-Baki, A.A.; Anderson, J.D. Vigour determination in soybean seed by multiple criteria. Crop Sci. 1973, 1, 630–633.

10.    Garzoli, S.; Orlando, F.; Iriti, M.; Vtalini, S. Solanum linnaeanum leaves: Chemical profiling of VOCs and effects on seed germination and early growth of monocots and dicots. Chem Biodivers. 2022, 19, e202100975.

11.    Vitalini, S.; Orlando, F.; Vaglia, V.; Bocchi, S.; Iriti, M. Potential role of Lolium multiflorum Lam. in the management of rice weeds. Plants 2020, 9, 324.

12.    Polatoğlu, K.; Karakoç, Ö.C.; Görenc, N. Phytotoxic, DPPH scavenging, insecticidal activities and essential oil composition of Achillea vermicularis, A. teretifolia and proposed chemotypes of A. biebersteinii (Asteraceae). Ind. Crops. Prod. 2013, 51, 35–45.

13.    Çakır, A.; Özer, H.; Aydın, T.; Kordali, Ş.; Çavuşoglu, A.T.; Akçin, T., Mete, E.; Akçin, A. Phytotoxic and insecticidal properties of essential oils and extracts of four Achillea species. Rec. Nat. Prod. 2016, 10, 154-167.

14.    Elshamy, A.; Abd-ElGawad, A.; Mohamed, T.; El Gendy, A.E.; Abd El Aty, A.A.; Saleh, I.; Moustafa, M.F.; Hussien, T.A.; Pare, P.W.; Hegazy, M. Extraction development for antimicrobial and phytotoxic essential oils from Asteraceae species: Achillea fragrantissima, Artemisia judaica and Tanacetum sinaicum. Flavour Fragr. J. 2021, 36, 352–364.

15.    Hatata, M.M.; El-Darier, S.M. Allelopathic potential of an aromatic plant; Achillea santolina L. on germination of Triticum aestivum L. Egypt. J. Exp. Biol. (Bot.) 2009, 5, 131-141.

16.    Tammam, A.A.; El-Bakatoushi, R.; El-Darier, S.M. The phytotoxic potential of Achillea santolina L. (Asteraceae) on Vicia faba L. and Hordeum vulgare L. Asia Life Sci. 2011, 20, 443-464.

17.    Pour, A.P.; Farahbakhsh, H. Allelopathic effect of Achillea (Achillea santolina) on germination and growth of crop plants. J. Agric. Sci. Technol. 2011, 1045-1053.

18.    Darier, S.M.; Tammam, A.A. Potentially phytotoxic effect of aqueous extract of Achillea santolina induced oxidative stress on Vicia faba and Hordeum vulgare. Rom. J. Biol. -Plant Biol. 2012, 57, 3-25.

19.    Vitalini, S.; Madeo, M.; Tava, A.; Iriti, M.; Vallone L.; Avato, P.; Cocuzza C.E.; Simonetti, P.; Argentieri, M.P. Chemical Profile, Antioxidant and Antibacterial Activities of Achillea moschata Wulfen, an Endemic Species from the Alps. Molecules 2016, 21, 830.

20.    Tava, A.; Iriti, M.; Vitalini, S. Composition and antioxidant activity of the essential oil from Achillea moschata Wulfen growing in Valchiavenna and Valmalenco (Italian Central Alps Int. J. Hortic. Sci. Technol. 2020, 7, 335-341.

21.    Argentieri, M.P.; Madeo, M.; Avato, P.; Iriti, M.; Vitalini, S. Polyphenol content and bioactivity of Achillea moschata from the Italian and Swiss Alps. Z. Naturforsch. 2020, 75c, 57-64.

22.    Chandler, R.F.; Hooper, S.N.; Harvey, M.J. Ethnobotany and phytochemistry of yarrow, Achillea millefolium, Compositae. Economic Bot. 1982, 36, 203–223.

23.    Ali, S.I.; Gopalakrishnan, B.; Venkatesalu, V. Pharmacognosy, phytochemistry and pharmacological properties of Achillea millefolium L.: A review. Phytother. Res. 2017, 31, 1140–1161.

24.    Agar, O.T.; Dikmen, M.; Ozturk, N.; Yilmaz, M.; Temel, H.; Turkmenoglu, F. Comparative studies on phenolic composition, antioxidant, wound healing and cytotoxic activities of selected Achillea L. species growing in Turkey. Molecules 2015, 20, 17976–18000.

25.    Venditti, A.; Guarcini, L.; Bianco, A.; Rosselli, S.; Bruno, M.; Senatore, F. Phytochemical analysis of Achillea ligustica All. from Lipari Island (Aeolian Islands). Nat. Prod. Res. 2016, 30, 912–919.

26.    Zengin, G.; Aktumsek, A.; Ceylan, R.; Uysal, S.; Mocan, A.; Guler, G.O.; Mahomoodally, M.F.; Glamočlija, J.; Ćirić, A.; Soković, M. Shedding light on the biological and chemical fingerprints of three Achillea species (A. biebersteinii, A. millefolium and A. teretifolia). Food Funct. 2017, 8, 1152–1165.

27.    Afshari, M.; Rahimmalek, M.; Miroliaei, M. Variation in polyphenolic profiles, antioxidant and antimicrobial activity of different Achillea species as natural sources of antiglycative compounds. Chem. Biodivers. 2018, 15, e1800075.

28.    Yilmaz, M.A.; Ertas, A.; Yener, I.; Akdeniz, M.; Cakir, O.; Altun, M.; Demirtas, I.; Boga, M.; Temel, H. A comprehensive LC-MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala. J. Pharm. Biomed. Anal. 2018, 154, 413–424.

29.    Lee, H.J.; Sim, M.O.; Woo, K.W.; Jeong, D.-E.; Jung, H.K.; An, B.; Cho, H.W. Antioxidant and antimelanogenic activities of compounds isolated from the aerial parts of Achillea alpina L. Chem. Biodivers. 2019, 16, e1900033.

30.    Dehsheikh, A.B.; Sourestani, M.M.; Dehsheikh, P.B.; Mottaghipisheh, J.; Vitalini, S.; Iriti, M. Monoterpenes: Essential oil components with valuable features. Mini Rev. Med. Chem. 2020, 20, 958–974.

31.    Okamoto, Y.; Yamaji, K.; Kobayashi, K. Allelopathic activity of camphor released from camphor tree (Cinnamomum camphora). Allelopathy J. 2011, 27, 123–132.

32.    Nishida, N.; Tamotsu, S.; Nagata, N.; Saito, C.; Sakai, A. Allelopathic effects of volatile monoterpenoids produced by Salvia leucophylla: Inhibition of cell proliferation and DNA synthesis in the root apical meristem of Brassica campestris seedlingsJ. Chem. Ecol. 2005, 31, 1187–1203.