Abstract

Worldwide attention on plant study has expanded, and a wealth of data has accumulated to demonstrate the enormous potential of medicinal plants employed in diverse traditional systems. In the traditional medical system, Flacourtia indica (FI) has been used to cure a variety of illnesses, including cancer, diabetes, hepatic disorders, and snakebites. Due to the presence of a variety of phytochemicals, including alkaloids, flavonoids, tannins, terpenoids, glycosides, it may have antioxidant and anti-inflammatory activity. Thus, this study was designed to assess the analgesic, anti-inflammatory and antipyretic effects of the ethanolic extract of Flacourtia indica. Flacourtia indica was extracted using 100% ethanol followed by assessing acute toxicity and doses selected for the studies were 500 and 1000 mg/kg body weight. Swiss albino mice of either sex weighing 25-30 gm and Sprague dawley rats weighing 180-200 gm were used in this study. Analgesic activity was evaluated by using acetic acid induced writhing test, formalin induced paw licking and hot plate test. Anti-inflammatory effect was assessed using xylene and croton oil induced ear edema test and carrageenan induced paw edema test. Also the antipyretic effect was investigated. The Flacourtia indica extract exhibited significant effect against pain in acetic acid test (p<0.01), formalin test (p<0.01) and insignificantly in hot plate test. Inflammation was reduced by FI extract in xylene test (p<0.05), croton oil test (p<0.01) and significantly reduced the paw edema (p<0.001) in carrageenan-induced paw edema test. In the yeast-induced antipyretic test, administration of Flacourtia indica at both doses significantly (p< 0.001) reduced pyrexia. Within these two doses, higher doses (1000 mg/kg body weight) had better aptitudes in the reduction of pain, inflammation and pyrexia. The FI extract shown strong analgesic, anti-inflammatory, and antipyretic capabilities, according to the study results, and more research is needed to assess these effects and the potential of the plants.

Keywords:

Medicinal plant, Flacourtia Indica, analgesic effect, anti-inflammatory effect, yeast induced pyrexia.

References

1.    Thirunethiran, K.; Varalakshmi, B.; Suguna Bai, J.; Gomathi, S. Effect of different doses of cucurbita pepo linn extract as an anti-inflammatory and analgesic nutraceautical agent on inflamed rats. Int. J. Phar. Res. Dev, 2011, 3(7), 60-78.

2.      Estakhr, J.; Sanchooli, N.; Najafi, S.H.; Javdan, N. Anti-Inflammatory Activity of Ethanolic Extract of Physalis alkekengi. Res. J. Pharm. Biol. Chem. Sci. 2011, 2(3), 421-425.

3.      Wohlmuth, H.; Oliver, C.; Nathan, P.J. A review of the status of Western herbal medicine in Australia. J. Herb. Pharmacother, 2002, 2, 33-46.

4.      http://www.floraofbangladesh.com/2020/05/boichi-or-madagascar-plum-flacourtia.html.

5.      Eramma, N.K. A Comprehensive Review on Pharmacology of Flacourtia Indica (BURM.F.) Merr. (governor’s Plum). Int. J. Pharm. Chem. Sci. 2016, 5(3).

6.      Kirtikar, K.R.; Basu, B.D. Indian Medicinal Plants. 3rd ed. Vol II, Singh and MP Singh Publications. India. 1998, 220.

7.      Kaou, A.M.; Leddet, V.M.; Canlet. C.; Debrauwer, L.; Hutterm, S. Antimalarial compounds from aerial parts of Flacourtia indica (Flacoutiaceae). J Ethnopharmacol. 2010, 130(2), 272-274.

8.      Pachute, P.A.; Tyagi, S.; Mishra, A.; Shakya, A.; Kumar, D.; Patel, V.K. Preliminary evaluation of Anticancer activity of Flacourtia indica Merr. Bot. Res. Int. 2011, 4(3),43-47

9.      Asad, B.H.H.M.; Murtaza, G.; Siraj, S.; Khan, A.S.; Azhar, S.; Hussain, S.M.; Ismail, T.; Hussain, S.M.; Hussain, I. Enlisting the scientifically unnoticed medicinal plants of Pakistan as a source of novel therapeutic agents showing anti-venom activity. Afr. J. Pharma. Pharmacol. 2011, 5(20), 2292-2305.

10.   Kim, J.S.; Ju, J.B.; Choi, C.W.; Kim, S.C.; Hypoglycemic and antihyperlipidemic effect of four Korean Medicinal plants in alloxan induced diabetes rats. Amer. J. Biochem. Biotech. 2006, 2(4), 154-160.

11.   Koster, R.; Anderson, M.; De-Beer, E.J. Acetic acid analgesic screening. Fed. Proc. 1959, 18, 412–417.

12.   Eddy, N.B.; Leimbach, D. Synthetic Analgesics II. Dithienylbutenyl- And Dithienylbutylamines. J. Pharmacol. Exp. Therap. 1953, 107(3), 385-93.

13.   Hunskaar, S.; Hole, K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain 1987, 30, 103-114.

14.   Vinegar, R.; Schreiber, W.; Hugo R, Biphasic development of carrageenan-induced oedema in rats. J. Pharmacol. Exp. Ther. 1969, 166, 96-103.

15.   Dai, Y.; Liu, L.H.; Kou, J.P.; Anti-inflammatory effect of aqueous extract of Wu-HU-Tang. China. Pharm. Uni. 1995, 6, 362-364.

16.   Swingle, K.F.; Shideman, F.E. Phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J. Pharmacol. Exp. Ther. 1972, 185, 226–234.

17.   Loux, J.J.; De Palma, P.D.; Yankell, S.L. Antipyretic testing of aspirin in rats. Toxicol. Appl. Pharmacol. 1972, 22, 672–675.

18.   Victor, B.O.; Caleb, O.W.; Ayodele, O. S.; Samuel, B.O. J. Ethnopharmacol. 2004, 90, 317-321.

19.   Vyklicky, L.Techniques for the study of pain in animals, In: Bonica, J.J.; Liebeskin & Albe-Fessard, D.G. (Eds), Advances in Pain Research and Therapy. Raven. New York, USA, 1979, 773-778.

20.   Vongtau, H.O.; Abbah, J.; Mosugu, O., et al. Antinociceptive profile of the ethanolic extract of Neorautanenia mitis root in rats and mice. J. ethnopharmacol. 2004, 92(2-3), 317- 324.

21.   Tjolsen, A.; Gerge, O.G.; Hunskaar, S., et al. The formalin test: an evaluation of method. Pain 1992, 51, 3-17.

22.   Ghannadi, A.; Hajhashemi, V.; Jafarabadi, H. An investigation of the analgesic and anti-inflammatory effects of Nigella sativa seed polyphenols. J. Medicinal. Food. 2005, 8, 488-493.

23.   Gabor, M. Mouse Ear Inflammation Models and their Pharmacological Applications. 2000, Akadémiai Kiadó, Budapest, Hungary.

24.   Halliwell, B.; Gutteridge, J.M.C. Free radicals in biology and medicine, 1999, Oxford University press.

25.   Conner, E.M.; Grisham, M.B. Inflammation, free radicals, and antioxidants. Nutrition 1996, 12(4), 274-277.

26.   Collier, H.O.; Dinneen, L.C.; Johnson, C.A.; Schneider, C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br. J.   Pharmacol. 1968, 32(2), 295–310.

27.   Deraedt, R.; Jouquey, S.; Delevallée, F.; Flahaut, M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur. J. Pharmacol. 1980, 61(1), 17-24.

28.   Roberts, L.J.; Morrow, J.D. Analgesic, antipyretic and Anti-inflammatory Agents and Drugs Employed in the Treatment of Gout. In: Eds. Goodman and Gilman’s The Pharmacological Basis of Therapeutics. 10th ed., McGraw-Hill. New York; 2001, 687-732.

29.   Richardson, Kilob, S.; Hargreavesab, K.M. Cannabinoids reduce hyperalgesia and inflammation via interaction with peripheral CB₁ receptors. Pain 1998, 75(1),111-119.

30.   Bensreti, M.M.; Sewell, R.D.E. Selective effects of dopaminergic modifiers on antinociception produced by different opioid receptoragonists. Pro. Br. Pharmacol. Soc. 1983, 6th – 8th July,70.

31.   Headley, P.M.; O’Shaughnessy, C.T. Evidence for opiate and dopamine interaction in striatum. Br. J. Pharmacol. 1985, 86 (pro. Suppl.), 700.

32.   Wigdor, S.; Wilcox, G.L. Central and systemic morphine-induced antinociception in mice: Contribution of descending serotonergic and noradrenergic pathways. J. Pharmacol. Exp. Ther. 1987, 242, 90-95.

33.   Pasero, C.; Paice, J.A.; McCaffery, M. Basic Mechanisms underlying the causes and effects of pain. In Pain, McCaffery M, Pasero C (eds). Mosby: St. Louis 1999, 15- 34.

34.   Salvemini, D.; Wang, Z.-Q.; David, M.B.; Michael, K.S.; Mark, G.C.; Pamela, T.M. Evidence of peroxynitrite involvement in the carrageenan-induced rat paw edema. Eur. J. Pharmacol. 1996, 303(3), 217-220.

35.   Loram, L.C.; Fuller, A.; Fick, L.G.; Cartmell, T.; Poole, S.; Mitchell, D. Cytokine Profiles During Carrageenan-Induced Inflammatory Hyperalgesia in Rat Muscle and Hind Paw. J. Pain 2007, 8, 127-136.

36.   Ribeiro, R.V.; Matos da Silva, R.; Joaquim Lima, J.C.S.; Martins, D.T.O. Antiinflammatory, antinociceptive and antipyretic effects of hydroethanolic extract from Macrosiphonia velame (A. St.-Hil.) M. Arg. in animal models. Braz. J. Pharm. Sci. 2010, 46(3), 515-523.

37.   DiMartino, M.J.; Campbell, J.R.G.K.; Wolff, C.E.; Hanna, N.; The pharmacology of arachidonic acid-induced rat paw edema. Agents and Actions 1987, 21 (3-4), 303-305.

38.   Luber-Narod, J.; Austin-Ritchie, T.; Hollins, C.; Menon, M.; Malhotra, R.K.; Baker, S.; et al. Urol. Res. 1997, 25, 395–9.

39.   Atta, A.H.; Alkofahi, A. Anti-nociceptive and anti-inflammatory effects of some Jordanian medical plant extracts. J. Ethnopharm. 1998, 60,117-124.

40.   Kavimani, S.; Vetrichelvan, T.; Ilango, R.; Jaykar, B. Antiinflammatory activity of the volatile oil of  Toddalia asiatica. Indian. J. Pharm. Sci. 1996, 58(2), 67–70.

41.   Simmons, D.L.; Botting, R.M.; Hla, T. Cyclooxygenase isozymes: the biology of prostaglandin synthesis and inhibition. Pharmacol. Rev. 2004, 56, 387-437.